ABTRACT: Baseflow estimation is important for hydrological activities such as water supply, irrigation, river transportation, energy production and groundwater practice. In this study, different methods widely used in the literature for baseflow estimation are applied on streams in the karstic Mediterranean Region. Three methods, the nonlinear baseflow separation, United Kingdom Institute of Hydrology and Recursive Digital Filter, were performed on daily flows of gauging stations in the region and results were compared. It is seen that the calculated baseflow is not far from results of previous case studies available for the study area. However, the nonlinear baseflow separation method is found to be the closest in terms of approaching the results of the previous studies.